细胞工程学

细胞工程学（32课时/2学分）

Cytotechnology (32 hours/2 credits)

课程描述：

细胞工程是细胞水平上的生物技术，包括植物细胞工程和动物细胞工程两部分内容。植物细胞工程主要讲授以下内容：植物细胞、组织和器官培养的发展历史和研究概况、植物细胞工程实验室的实验装备和常用仪器设备、无菌操作技术、植物培养基的组成和配制方法、愈伤组织的诱导和培养技术、通过愈伤组织诱导产生根和芽以至再生植株的培养技术、植物单细胞悬浮培养技术、胚状体的诱导和培养技术、发根的培养技术及其应用、离体芽的快繁技术、花药和花粉培养技术、原生质体的分离、纯化和培养技术以及细胞融合与体细胞杂交技术、种质资源的低温保存技术、植物次生代谢产物的生产和应用、植物基因转移技术和转基因植物的制备与应用等。动物细胞工程主要讲授以下内容：动物细胞培养发展简史、体外培养细胞的生物学特性（包括细胞形态、贴壁和生长曲线）、动物细胞培养常用仪器设备、动物细胞培养基的组成、制备与灭菌、无血清培养基、动物细胞的原代培养和传代培养、细胞系、细胞系的转化、鉴定、污染及其冷冻保存、细胞的克隆与筛选、细胞融合与杂交技术、杂交瘤技术和单克隆抗体的生产技术、细胞毒性、细胞核重编程、胚胎干细胞和诱导性胚胎干细胞（iPS）技术、3D细胞培养技术（包括类器官培养、3D打印器官和细胞培养肉）、动物细胞的基因转染技术、动物基因转移技术与转基因动物的制备、基因编辑技术（包括TALEN、CRISPR/Cas9、单碱基编辑和 NgAgo-gDNA）等技术的原理与应用、克隆动物的制备等。

Cytotechnology is a kind of biotechnology at cellular level. It can be divided into two parts of plant and animal cytotechnology. For plant cytotechnology, the following contents will be introduced including: the history of plant cell, tissue and organ culture, laboratory facilities often used, aseptic techniques, media composition and preparation, initiation and maintenance of callus, induction of organogenesis and somatic embryogenesis, suspension culture of dispersed plant cells, in vitro culture and applications of hairy roots, anther and pollen culture, micropropagation by bud proliferation, isolation, purification and culture of protoplasts, protoplast fusion and somatic hybridization, cryopreservation of germplasm, the principle and protocols of often used gene transfer methods, Production and use of transgenic plants, etc. For animal cytotechnology, the following contents will be introduced including: the history of animal cell culture, biological features of cultured cells including morphology, attachment and growth curve, facilities and equipment used in animal cell culture, components of animal cell medium, preparation and sterilization of medium, serum-free medium, primary cell culture and subculture, cell line, transformation of cultured cells, characterization of cell lines, contamination of cultured cells, cryopreservation of cultured cells, cell cloning and selection, animal cell fusion and hybridization, production of hybridoma and monoclonal antibody, cytotoxicity, embryonic stem cells, nuclear reprogramming and induced pluripotent stem cells, 3D cell culture technology (including organoid culture, 3D-printed organ and cell-cultured meat), technical principles and protocols of animal cell transfection, transgenic animals, genome editing technologies (like TALEN, CRISP/Cas9, single base-editing and NgAgo-gDNA), and production of cloned animals, etc.