分子生物学实验

分子生物学实验（32课时/1学分）

Molecular Biology Practical (32 hours/1 credit)

课程描述： 

本课程是以生物化学实验和微生物学实验为基础，综合并发展出的分子生物学实验技术体系，该技术体系是生命科学相关专业进行研究和应用的现代生物学技术。本课程是生物科学、生物技术、生态学专业的必修课，主要介绍分子生物学的基本操作技术与实验手段，包括基因组DNA的提取与鉴定、质粒DNA的提取与鉴定、总RNA的提取与鉴定、PCR扩增、限制性内切酶消化、分子克隆全过程和基因文库的构建等实验项目的原理及步骤。通过课程学习和操作实践，要求学生掌握分子生物学实验的基本原理和操作技能，并能够利用这些技术分析和解决分子生物学相关领域的实际问题。

This course is a system of molecular biology experiment technology based on biochemistry experiments and microbiology experiments, which is a modern biological technology for research and application of life science. This course is a compulsory course for students of Biology, Biotechnology, and Ecology. The course includes the basic operational techniques and experimental means of molecular biology, including Principles and procedures of experimental projects such as genomic DNA extraction and identification, plasmid DNA extraction and identification, total RNA extraction and identification, PCR amplification, restriction endonuclease digestion, the process of molecular cloning and construction of gene library. Through the course study and operation practice, students are required to master the basic principles and operation skills of molecular biology experiments, and to be able to analyze and solve practical problems in related fields of molecular biology with these techniques.