王媛媛

一、个人简介

姓名：王媛媛

学历：博士研究生

职称：副教授

办公电话：0532-82032081

邮箱：wangyuanyuan@ouc.edu.cn

二、在海德教授课程名称

分子生物学实验

三、研究方向

主要从事表观遗传学研究，以单细胞真核模式生物--嗜热四膜虫（Tetrahymena thermophila）为研究体系，研究内容主要包括：1）DNA N⁶-腺嘌呤甲基化（6mA）酶复合体的功能和调控机制；2）真核生物6mA的功能演化研究。研究成果相继发表在PNAS、Nucleic Acids Research、Genome Research、Science China Life Sciences等国际主流期刊。

四、研究成果

1. Lili Duan^#, Haicheng Li^#, Aili Ju, Zhe Zhang, Junhua Niu, Yumiao Zhang, Jinghan Diao, Yongqiang Liu, Ni Song, Honggang Ma, Kensuke Kataoka, Shan Gao*, Yuanyuan Wang* (通讯) (2025). Methyl-dependent auto-regulation of the DNA N⁶-adenine methyltransferase AMT1 in the unicellular eukaryote Tetrahymena thermophila. Nucleic Acids Research, 53 (3), gkaf022. DOI: 10.1093/nar/gkaf022. (SCI, IF=16.6)

2. Yuanyuan Wang (一作和共同通讯) ^# *, Bei Nan^#, Fei Ye^#, Zhe Zhang^#, Wentao Yang^#, Bo Pan, Fan Wei, Lili Duan, Haicheng Li, Junhua Niu, Aili Ju, Yongqiang Liu, Dantong Wang, Wenxin Zhang, Yifan Liu*, Shan Gao* (2025). Dual modes of DNA N⁶-methyladenine maintenance by distinct methyltransferase complexes. Proceedings of the National Academy of Sciences of the United States of America, 122 (3), e2413037121. DOI: 10.1073/pnas.2413037121. (SCI, IF=9.4)

3. Yalan Sheng^#, Yuanyuan Wang^# (共同一作), Wentao Yang^#, Xue Qing Wang, Jiuwei Lu, Bo Pan, Bei Nan, Yongqiang Liu, Fei Ye, Chun Li, Jikui Song, Yali Dou, Shan Gao, Yifan Liu (2024). Semi-conservative transmission of DNA N⁶-adenine methylation in a unicellular eukaryote. Genome Research, 34:740-756. DOI: 10.1101/gr.277843.123. (SCI, IF=6.2)

4. Bo Pan^#, Fei Ye^#, Tao Li, Fan Wei, Alan Warren, Yuanyuan Wang (通讯), Shan Gao (2023). Potential role of N⁶-adenine DNA methylation in alternative splicing and endosymbiosis in Paramecium bursaria. iScience, 26(5), 106676. DOI: 10.1016/j.isci.2023.106676. (SCI, IF=4.6)

5. Fan Wei, Bo Pan, Jinghan Diao, Yuanyuan Wang* (共同通讯作者)，Yalan Sheng* & Shan Gao (2022). The micronuclear histone H3 clipping in the unicellular eukaryote Tetrahymena thermophila. Marine Life Science & Technology, 4，584-594. DOI: 10.1007/s42995-022-00151-0. (SCI, IF=5.7)

6. Yong Chi^#, Xiangrui Chen^#, Yuqing Li, Chundi Wang, Tengteng Zhang, Alex Ayoub, Alan Warren, Weibo Song & Yuanyuan Wang (通讯) (2021). New contributions to the phylogeny of the ciliate class Heterotrichea (Protista, Ciliophora): analyses at family-genus level and proposed new evolutionary hypotheses. Science China Life Sciences, 64(4), 606-620. DOI: 10.1007/s11427-020-1817-5. (SCI, IF=10.372) 

7. Lili Duan^#, Ting Cheng^#, Fan Wei, Yu Qiao, Congcong Wang, Alan Warren, Junhua Niu  & Yuanyuan Wang (通讯) (2021). New contribution to epigenetic studies: Isolation of micronuclei with high purity and DNA integrity in the model ciliated protist, Tetrahymena thermophila. European Journal of Protistology, 80, 125804. DOI: 10.1016/j.ejop.2021.125804. (SCI, IF=3.471) 

8. Xiaoteng Lu^#, Yuanyuan Wang^# (共同一作), Saleh A. Al-Farraj, Hamed El-Serehy, Jie Huang & Chen Shao (2020). The insights into the systematic relationship of Gastrostyla-affinitive genera, with report on a new saline soil ciliate genus and new species (Protozoa, Ciliophora). BMC Evolutionary Biology, 20(1), 92. DOI: 10.1186/s12862-020-01659-8. (SCI, IF=3.26) 

9. Yuanyuan Wang (一作) ^#, Yalan Sheng^#, Yongqiang Liu^#, Wenxin Zhang, Ting Cheng, Lili Duan, Bo Pan, Yu Qiao, Yifan Liu & Shan Gao (2019). A distinct class of eukaryotic MT-A70 methyltransferases maintain symmetric DNA N⁶-adenine methylation at the ApT dinucleotides as an epigenetic mark associated with transcription. Nucleic Acids Research, 47(22), 11771-11789. DOI: 10.1093/nar/gkz1053. (SCI, IF=11.5)

10. Ting Cheng^#, Yuanyuan Wang^# (共同一作), Jie Huang^#, Xiao Chen^#, Xiaolu Zhao^#, Shan Gao & Weibo Song (2019). Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila. Marine Life Science & Technology, 1, 4-14. DOI: 10.1007/s42995-019-00015-0. (SCI, IF=5.0)

11. Yuanyuan Wang (一作) ^#, Xiao Chen^#, Yalan Sheng, Yifan Liu & Shan Gao (2017). N⁶-adenine DNA methylation is associated with the linker DNA of H2A.Z-containing well-positioned nucleosomes in Pol II-transcribed genes in Tetrahymena. Nucleic Acids Research, 45(20), 11594-11606. DOI: 10.1093/nar/gkx883. (SCI, IF=11.5) 

12. Yuanyuan Wang (一作), Yalan Sheng, Yongqiang Liu, Bo Pan, Jie Huang, Alan Warren & Shan Gao (2017). N⁶-methyladenine DNA modification in the unicellular eukaryotic organism Tetrahymena thermophila. European Journal of Protistology, 58, 94-102. DOI: 10.1016/j.ejop.2016.12.003. (SCI, IF=2.4) 

13. Xiaolu Zhao^#, Yuanyuan Wang^# (共同一作), Yurui Wang^#, Yifan Liu & Shan Gao (2017). Histone methyltransferase TXR1 is required for both H3 and H3. 3 lysine 27 methylation in the well-known ciliated protist Tetrahymena thermophila. Science China Life Sciences, 60(3), 264-270. DOI: 10.1007/s11427-016-0183-1. (SCI, IF=3.085)